Implemented kill switches: 1
Kill Switch 1
Class
Induced quorum sensing leads to toxic expression
Parallel circuits for holin/antiholin system
Toxins
| Name |
Number |
Comment |
| ccdB |
|
Blocks DNA polymerases to bind to DNA through reducing gyrase |
| holin |
|
|
Antagonists
| Name |
Number |
Comment |
| antiholin (LrgA and LrgB) |
|
|
Topology
Parts
| Name |
Number |
Comment |
| IPTG |
|
Control concentration and prevent overgrowth; see paper |
| pLac |
|
Control concentration and prevent overgrowth; see paper |
| LuxR |
|
|
| LuxI |
|
|
| AHL |
|
|
| pLuxR |
|
|
| pLuxR |
|
Prevent horizontal gene transfer; see paper |
| CheZ |
|
Prevent horizontal gene transfer; see paper |
| RFP |
|
Prevent horizontal gene transfer; see paper |
| consecutive promoter |
|
|
| IrgB |
|
|
| IrgA |
|
|
| pLuxR |
|
|
| trzN |
|
|